Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries
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چکیده
منابع مشابه
Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries
A key limitation in using heterologous genomic or metagenomic libraries in functional genomics and genome engineering is the low expression of heterologous genes in screening hosts, such as Escherichia coli. To overcome this limitation, here we generate E. coli strains capable of recognizing heterologous promoters by expressing heterologous sigma factors. Among seven sigma factors tested, RpoD ...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2015
ISSN: 2041-1723
DOI: 10.1038/ncomms8045